Mar 3 : The 'ins" and 'outs' of Nerve Growth Factor signaling - a dynamic view
Speaker：Brian B. RUDKIN, PhD
CNRS/Ecole Normale Supérieure de Lyon & East China Normal University, Shanghai
Host: Prof. Jiemin Wong
When: 2014-3-3 10:00
Where: Conference Room 534, School of Life Sciences
Abstract: Nerve growth factor (NGF) signal transduction has been studied using numerous approaches. This presentation will go off the beaten track somewhat, to illustrate not only the beauty, but above all the informative nature of time-lapse confocal fluorescence microscopic analysis of NGF receptor trafficking. Biochemical, molecular and microscopic analysis of wild type and mutated receptor, combined with ligand distribution offer insight into the trafficking and activation of the NGF receptor, TrkA. A brief introduction to the use of novel combinatorial proteins for investigation signaling pathways and cellular processes will be presented.
Related Publications:
NGF signaling and receptor traffic
Com, E., Lagadec, C., Page, A., El Yazidi-Belkoura, I., Slomianny, C., Spencer, A., Hammache, D., Rudkin, B. B. and Hubert Hondermarck. (2007). TrkA signaling in breast cancer cells involves the DNA-dependent protein kinase subunit Ku70 to prevent apoptosis. Mol Cell Proteomics 6, 1842-54. E-Pub 2007 July 7
Jullien, J., Guili, V., Derrington, E. A., Darlix, J. L., Reichardt, L. F., and Rudkin, B. B. (2003). Trafficking of TrkA-green fluorescent protein chimera during nerve growth factor -induced differentiation. J Biol Chem 278, 8706-8716.
Jullien, J., Guili, V., Reichardt, L. F., and Rudkin, B. B. (2002). Molecular Kinetics of Nerve growth factor receptor trafficking and activation. J Biol Chem 277, 38700-38708.
Urdiales, J.L., Becker, E., Andrieu, M., Thomas, A., Jullien, J., van Grunsven, L.A., Menut, S., Evan, G.I., Martín-Zanca, D. and Rudkin, B.B. (1998). Cell cycle phase-specific surface expression of Nerve Growth Factor receptors TrkA and p75NTR . J. Neurosci 18:6767-6775.
Rudkin, B.B., Lazarovici, P., Levi, B.Z., Abe, Y., Fujita, K., and Guroff, G. (1989). Cell cycle-specific action of nerve growth factor in PC12 cells : Differentiation without proliferation. EMBO J. 8: 3319-3325.
Peptide Aptamers
Dibenedetto S*, Cluet D*, Stebe PN, Baumlé V, Bickle M, De Chassey, Mikaelian, I, Colas, P, Spichty M, Zoli M, Rudkin B.B. (2013). Calcineurin A vs NS5A-TP2/HD Domain Containing Protein 2: a case study of site-directed low-frequency random mutagenesis for dissecting target specificity of peptide aptamers. Mol Cell Proteomics 12:1939-52. Epub 2013 Apr 10.