We provides top-quality, high-titer retroviral and lentiviral packaging services at great prices with turnaround time as quick as four weeks. Save yourself the time and effort on virus preparation or even DNA vector design and construction, and receive ready-to-transduce retroviral and lentiviral particles.
◆Service Features
Affordable prices and rapid turnaround.
High-titer lentivirus.
Safer viral packaging system is provided.
Open-access DNA vector design platform.
Precise and strictly controlled titer measurements performed by the fluorescence method .
The client will receive 1 mL retrovirus or lentivirus.
◆Description of Services
Custom construction of retrovirus and lentivirus
The customer may simply provide us with the names of the gene and the promoter that they wish to insert into the basic viral vector, and we will custom construct the vector. This work may be performed as part of a packaging service or as a stand-alone service.
◆Vector provided
TableⅠ：Retropack PT67 system |
Vector | Virus | Promoter | Transient Virus Production or Stable Virus Production | Envelope | Target cells | Application |
PLNCX2 | MMLV | CMV | stable | 10A1 | Mouse, rat, hamster Mink, cat, dog, monkey, human | Over expression |
pRIGHT16c | MMLV | U6 and H1 | stable | 10A1 | Mouse, rat, hamster Mink, cat, dog, monkey, human | RNA interference |
TableⅡ：Retropack 293T system* |
Vector | Virus | Promoter | Transient Virus Production or Stable Virus Production | Envelope | Target cells | Application |
PLNCX2
| MMLV | CMV | transient | VSV-G | Mouse, rat, hamster Mink, cat, dog, monkey, human, avian, fish, insect | Over expression |
pRIGHT16c | MMLV | U6 and H1 | transient | VSV-G | Mouse, rat, hamster Mink, cat, dog, monkey, human avian, fish, insect | RNA interference |
*:Add helper vector; VSV-G and gag-pol
TableⅢ：Lentipack 293T system* |
Vector | Virus | Promoter | Transient Virus Production or Stable Virus Production | Envelope | Target cells | Application |
GIPZ lentiviral shRNAmir | HIV | U6 | transient | VSV-G | Mouse, rat, hamster Mink, cat, dog, monkey, human, avian, fish, insect | RNA interference |
pLVX-IRES-ZsGreen1
| HIV | CMV | transient | VSV-G | Mouse, rat, hamster Mink, cat, dog, monkey, human, avian, fish, insect | Over expression |
pLL3.7 | HIV | CMV | transient | VSV-G | Mouse, rat, hamster Mink, cat, dog, monkey, human, avian, fish, insect | RNA interference |
*:Add helper vector: pSPAX2 and pMD2G
◆Titer Measurement
Because all of our vectors encode a fluorescence protein gene, so we use fluorescence method measure the virus titer. This method can only determine live virus, so it is more precise.
◆Successful Cases of Transduction
We aim to offer customers top quality and high transduction rate retrovirus and lentivirus. Several successful cases are as follows.
These pictures show that essentially 100% of the cells are transduced by our virus.
Retrovirus infect ES cells
Lentivirus infect U2OS cells.
Lentivirus infect AGS cells
Lentivirus infect SW620
