Molecular Oncology Laboratory
Chuangui Wang
Education & Training
Instructor: College of Medicine, University of South Florida. 2006.
Postdoctoral Fellow, Interdisciplinary Oncology Program, H Lee Moffitt Cancer Center, USA. 2001-2005.
Research Assistant: Department of Applied Biology and Chemistry, Hong Kong Polytechnic University. 2000.
PhD, Biomedical Engineering, University of Central China University of Science and Technology. 2000.
Research Interests
Our laboratory mainly focuses on studying the basic biology of signal transduction and gene regulation. Currently, we aim to clarify the mechanisms by which a number of proteins control different cellular regulatory processes, such as gene expression, protein degradation, cell division, DNA damage response and autophagy, with aims to identify novel potential anti-tumor targets and drugs.
Publications
1. Roles of the KRAB-associated co-repressor KAP1 S473 phosphorylation in DNA damage response. Chen Hu, Shengping Zhang, Xuan Gao, Xiaojing Gao, Xiaohong Xu, Ya Lv, Yan Zhang, Zhenhong Zhu, Changqing Zhang, Qiao Li, Jiemin Wong, Yongping Cui, Wen Zhang, and Chuangui Wang*. Journal of Biological Chemistry, 2012 287: 18937-18952.
2. Tyrosines 303/343/353 within the Sprouty-related domain of Spred2 are essential for its interaction with p85 and inhibitory effect on Ras/ERK activation. Songshu Meng, Miao Zhang, Wenyan Pan, Zhongliang Li, Deborah H. Anderson, Shengping Zhang, Baoxue Ge, Chuangui Wang*. Int J Biochem Cell Biol, 2012. 44:748-58.
3. Sensitive electrochemical immunosensor for α-Synuclein based on dual signal amplification using PAMAM dendrimer-encapsulated Au and enhanced gold nanoparticle labels. Yarui An, Xiaoli Jiang, Wenji Bi, Hua Chen, Litong Jin, Shengping Zhang, Chuangui Wang*, Wen Zhang. Biosensors and Bioelectronics， 2012. 32: 224-30.
4. Phosphorylation of Mps1 by BRAF(V600E) prevents Mps1 degradation and contributes to chromosome instability in melanoma. Liu J, Cheng X, Zhang Y, Li S, Cui H, Zhang L, Shi R, Zhao Z, He C, Wang C, Zhao H, Zhang C, Fisk HA, Guadagno TM, Cui Y. Oncogene, 2012. doi: 10.1038/onc.2012.94.
5. P14ARF inhibits the functions of adenovirus E1A oncoprotein. Jia Shen, Shengping Zhang, Yang Li, Wen Zhang, Jiandong Chen, Mary Zhang, Ting Wang, Ling Jiang, Xiuqun Zou, Jiemin Wong, Xiaotao Li, Yongping Cui, Chuangui Wang*. Biochemical Journal, 2011. 434: 275-285.
6. Lectin-based electrochemical biosensor constructed by functionalized carbon nanotubes for the competitive assay of glycan expression on living cancer cells. Xinai Zhang, Yingqiao Teng, Ying Fu, Shengping Zhang, Ting Wang, Chuangui Wang, Litong Jin and Wen Zhang. Chemical Science, 2011. 2: 2353-60.
7. Association of Shp2 with phosphorylated IL-22R1 is required for Interleukin-22-induced MAP kinase activation. Songshu Meng, Qiu Gui, Qing Xu, Kaixin Lu, Xinan Jiao, Jian Fan, Baoxue Ge, Yuehai Ke, Shengping Zhang, Jie Wu, and Chuangui Wang*. Journal of Molecular Cell Biology, 2010. 2: 223-230.
8. Degradation of the human mitotic checkpoint kinase Mps1 is cell-cycle regulated by APC/cCdc20 and APC/cCdh1 ubiquitin ligases. Yongping Cui, Xiaolong Cheng, Shujing Li, Ce Zhang, Chuangui Wang, Thomas M. Guadagno. Journal of Biological Chemistry, 2010. 285: 32988-32998.
9. Lectin-Based Biosensor Strategy for Electrochemical Assay of Glycan Expression on Living Cancer Cells. Xinai Zhang, Yingqiao Teng, Ying Fu, Lili Xu, Shengping Zhang, Bei He, Chuangui Wang (correspondence author), Wen Zhang. Analytical Chemistry, 2010. 82:9455-60.
10. A Photoelectrochemical Immunosensor Based on Au-Doped TiO2 Nanotube Arrays for the Detection of α-Synuclein. Yarui An, Linlin Tang, Xiaoli Jiang, Hua Chen, Meicheng Yang, Litong Jin, Shengping Zhang, Chuangui Wang, Wen Zhang. Chemistry-A European Journal, 2010. 16: 14439-46.
11. Triterpenoids and Steroids from the Fruits of Melia toosendan and Their Cytotoxic Effects on Two Human Cancer Cell Lines. Shi-Biao Wu, Jing-Jing Su, Lian-Hui Sun, Wen-Xuan Wang, Yun Zhao, Hui Li, Sheng-Ping Zhang, Guang-Hui Dai, Chuan-Gui Wang, and Jin-Feng Hu. Journal of Natural Products, 2010. 73: 1898–1906.
12. REG modulates p53 activity by regulating its cellular localization. Jian Liu, Guowu Yu, Yanyan Zhao, Dengpan Zhao, Ying Wang, Lu Wang, Jiang Liu, Lei Li, Yu Zeng, Yongyan Dang, Chuangui Wang, Guang Gao, Weiwen Long, David M. Lonard, Shanlou Qiao, Ming-Jer Tsai, Bianhong Zhang, Honglin Luo, and Xiaotao Li. Journal of Cell Science, 2010. 123:4076-4084.
13. Gynostemosides A–E‚ megastigmane glycosides from Gynostemma pentaphyllum. Zhen Zhang‚ Wei Zhang‚ Yan-Ping Ji‚ Yun Zhao‚ Chuan-Gui Wang‚ Jin-Feng Hu. Phytochem‚ 2010. 71: 693-700.
14. Inhibition of SUV39H1 Methyltransferase Activity by DBC1. Zhenyu Li‚ Lihong Chen‚ Neha Kabra‚ Chuangui Wang‚ Jia Fang‚ Jiandong Chen. Journal of Biological Chemistry, 2009. 284:10361-66.
15. SirT1 Is an Inhibitor of Proliferation and Tumor Formation in Colon Cancer. Neha Kabra‚ Zhenyu Li‚ Lihong Chen‚ Baozong Li‚ Xiaohong Zhang‚ Chuangui Wang‚ Timothy Yeatman‚ Domenico Coppola‚ Jiandong Chen. Journal of Biological Chemistry, 2009. 284: 18210-17.
16. KAP1 contributes to the negative regulation of E2F1 in the absence of pRb. Chuangui Wang‚ Lihong Chen‚ Yihong Ma‚ W. Douglas Cress and Jiandong Chen. Journal of Biological Chemistry, 2007. 282: 29902-09.
17. Interactions between E2F1 and SirT1 regulate apoptotic response to DNA damage. Chuangui Wang‚ Lihong Chen‚ Xinghua Hou‚ Zhenyu Li‚ Neha Kabra‚ Yihong Ma‚ Shino Nemoto‚ Toren Finkel‚ Wei Gu‚ W. Douglas Cress and Jiandong Chen. Nature Cell Biology, 2006. 8: 1025-31.
18. Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism. Journal of Biological Chemistry. Chuangui Wang‚ Hou X‚ Mohapatra S‚ Ma Y‚ Cress WD‚ Pledger WJ‚ Chen J. Journal of Biological Chemistry, 2005. 280: 12339-43.
19. MDM2 interaction with nuclear corepressor KAP1 contributes to p53 inactivation. Chuangui Wang‚ Ivanov A‚ Chen L‚ Fredericks WJ‚ Seto E‚ Rauscher FJ‚ Chen J. The EMBO Journal, 2005. 24: 3279-90.