报告题目：Sensing and mapping DNA methylation

报告人：Prof. Matthias Bochtler, Institute of Biochemistry and Biophysics (IBB) of the Polish Academy of Sciences

主持人：翁杰敏教授

报告时间：2019年10月14日10:00（周一上午）

报告地点：生命科学学院534小会议室

报告人简介：

1990–1992：Studies in physics at the LMU Munich

1992–1993：Guest student at the University of Cambridge, UK

1993–1995：Studies in physics at the LMU Munich. Diploma with distinction

1996–1999：PhD at the Max Planck Institute of Biochemistry in Martinsried (Germany) under Prof. Robert Huber (“summa cum laude”)

1999–2000：Postdoc at the Max Planck Institute of Biochemistry in Martinsried

2001-present：Head of a joint MPG-PAN Junior Research Group at the International Institute of Molecular and Cell Biology (IIMCB) in Warsaw

2005/2006 Habilitation in biochemistry at the Institute of Bioorganic Chemistry (IChB) PAN in Poznan (submission 2005, official confirmation 2006).

2007-2011：Head of Structural Biology, Cardiff University (United Kingdom)

2009：Professorial title awarded by the President of the Republic of Poland

2011-present Group Leader at IIMCB and professor at the Institute of Biochemistry and Biophysics (IBB) of the Polish Academy of Sciences

报告内容：

Enzymatic DNA methylation comes in three main varieties, as as 5-methylcytosine, 4-methylcytosine, and 6-methyladenine. In the first part of my talk, I will report crystallographic and bioinformatic data on how DNA methylation is sensed by proteins, both negatively and positively. I will show that there is a small modular toolkit for this task, and that cytosine and adenine methylation are detected rather differently. In the second part of my talk, I will describe our efforts to characterize maintenance of DNA methylation in a model organism, the plant A. thaliana, by bisulfite conversion and reversible terminator (Illumina) sequencing, and by Nanopore sequencing. The data indicate strong long range correlations in methylation of a single DNA molecule, and provide evidence for crosstalk between different methylation types (CpG, CHG, CHH).